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    1. Cell Cycle/DNA Damage
    2. DNA/RNA Synthesis

    DNA/RNA Synthesis

    RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

    Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

    First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

    DNA/RNA Synthesis 相关产品 (63):

    Cat. No. Product Name Effect Purity
    • HY-12320
      Cycloheximide Inhibitor 99.45%
      Cycloheximide (Naramycin A) 是真核生物蛋白质合成的抑制剂,抑制体内蛋白质合成和RNA合成的 IC50 值分别为532.5 nM 和 2880 nM。
    • HY-17559
      Actinomycin D Inhibitor 99.89%
      Actinomycin D 抑制 DNA 修复,IC50 为 0.42 μM。
    • HY-13316
      Mitomycin C Inhibitor 98.89%
      Mitomycin C是一种抗肿瘤药物和抗生素,可有效的抑制DNA合成 (DNA synthesis)。
    • HY-17026
      Gemcitabine Inhibitor 99.92%
      Gemcitabine (NSC 613327;LY188011) 是一种 DNA合成 抑制剂,抑制 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1细胞生长的 IC50 分别为 37.6, 42.9, 92.7, 89.3 和 131.4 nM。
    • HY-13753
      Streptozocin Inhibitor 99.58%
      Streptozocin是一种有效的 DNA甲基化 试剂,在HL60,K562和C1498细胞中的IC50 分别为11.7,904 和 1024 μg/mL。
    • HY-N1584A
      Halofuginone hydrobromide Inhibitor 99.94%
      Halofuginone hydrobromide (RU-19110 hydrobromide) 是 Febrifugine 的一种低毒性衍生物,可从 Dichroa febrifuga 中分离出来。Halofuginone 是一种 ATP 竞争性的脯氨酰-tRNA 合成酶 (prolyl-tRNA synthetase) 抑制剂,Ki 为 18.3 nM。Halofuginone 通过抑制 TGF-β 活性可减轻骨关节炎。
    • HY-14776
      Quarfloxin Inhibitor
      Quarfloxin (CX 3543) 是一种具有抗肿瘤活性的氟喹诺酮类衍生物,其靶向抑制RNA pol I的活性,在神经母细胞瘤中的IC50 值在纳摩尔级别。Quarfloxin 干扰核糖体DNA中核蛋白与G-四重DNA结构的相互作用。
    • HY-112803
      GNE-371 是一种针对人类转录起始因子 TFIID 亚基 1 和类转录起始因子 TFIID 亚基 1 的第二溴端结构域的高效选择性化学探针,对 TAF1(2) 的 IC50 值为10 nM。
    • HY-19610
      alpha-Amanitin Inhibitor 99.79%
      alpha-Amanitin是几种致命毒蘑菇的主要毒素,通过抑制 RNA聚合酶II 发挥其毒性作用。
    • HY-17565
      Bleomycin sulfate Inhibitor >98.0%
      Bleomycin sulfate是具有有效抗肿瘤活性的DNA合成抑制剂。
    • HY-B0003
      Gemcitabine Hydrochloride Inhibitor 99.93%
      Gemcitabine hydrochloride是一种DNA合成抑制剂,在131.4 nM -2,PANC-1,PL-45和AsPC-1细胞中的 IC50 分别为37.6,42.9,92.7,89.3 和 131.4 nM。
    • HY-17393
      Carboplatin Inhibitor 99.88%
      Carboplatin (NSC 241240) 是一种 DNA 合成 抑制剂,其与 DNA 结合,抑制复制和转录并诱导细胞死亡。Carboplatin (NSC 241240) 是顺铂的衍生物,是一种有效的抗癌剂。
    • HY-18982
      Anisomycin Inhibitor 98.20%
    • HY-12742
      SCR7 Inhibitor 99.75%
    • HY-13323
      CX-5461 Inhibitor 98.47%
      CX-5461是一种有效,口服的rRNA合成抑制剂。 它在HCT-116,A375和MIA PaCa-2细胞中抑制RNA聚合酶I驱动的rRNA转录,IC50为142,113和54 nM。
    • HY-B0016
      Capecitabine Inhibitor 99.97%
      Capecitabine 是一种可用于口服的前药,可由胸苷磷酸化酶催化转变为其活性代谢物 Fluorouracil (FU)。
    • HY-B0313
      Hydroxyurea Inhibitor >98.0%
    • HY-19620
      Branaplam Agonist 99.67%
      Branaplam (LMI070) 是一种高效,选择性和口服活性的小分子 SMN2 剪接调节剂。
    • HY-19931
      COH29 Inhibitor 98.04%
      COH29是一种高效,具有抗癌活性的核糖核苷酸还原酶 (RNR) 抑制剂。抑制核糖核苷酸还原酶的 IC50 为16 μM。
    • HY-15457
      Triciribine Inhibitor 99.20%
      Triciribine 是一种 DNA 合成 (DNA synthesis) 抑制剂,也抑制 AktHIV-1/2IC50 分别为 130 nM 和 0.02-0.46 μM。
    Isoform Specific Products

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